The growing emergence of synthetic opioids in the nitazene subclass such as butonitazene poses a significant challenge for doping control and toxicological monitoring. Butonitazene is a highly potent synthetic opioid, structurally distinct from classical opioids; its potential misuse in competitive events such as horse and camel racing cannot be overlooked. The in vitro metabolism of butonitazene was studied using camel liver, and findings were compared with metabolic profiles obtained from Cunninghamella elegans.